376 papers
This study demonstrates that the histone chaperone FACT is essential for maintaining active promoter topology by preserving nucleosome integrity to prevent the invasion of chromatin-binding factors, thereby ensuring both local nanoscale domains and large-scale chromatin architecture.
This study identifies ZNF121 as a novel cytoplasmic cofactor that recruits YTHDF2 to target mRNAs, thereby enhancing m6A-independent mRNA decay and regulating cell-cycle progression and oncogene expression.
This paper introduces the amyloid packing difference (APD), a metric that quantifies structural variations between amyloid filaments based on unique cross-beta interactions and side chain orientations, demonstrating its ability to effectively distinguish disease-associated protofilament folds of proteins like alpha-synuclein and tau through clustering and specific value thresholds.
This study reveals that p63 and p73 form heteromeric complexes to co-occupy distal enhancers in cutaneous squamous cell carcinoma, where they cooperatively drive shared proliferative programs while exerting factor-specific roles—p63 reinforcing epithelial lineage and p73 supporting DNA replication and stress responses—ultimately sustaining tumorigenesis through a feed-forward EGFR signaling module dominated by Amphiregulin.
This study identifies PARP6 as a cardioprotective enzyme that suppresses cardiac fibrosis by ADP-ribosylating vimentin to inhibit RhoA-mediated actin stress fiber formation and myofibroblast activation.
By combining Vector-Enabled Metagenomics with Rolling Circle Amplification, researchers detected the beet curly top Iran virus (BCTIV) in European insects for the first time and confirmed its ability to infect watermelon and zucchini, demonstrating the effectiveness of this approach for early monitoring of emerging geminiviruses.
This study characterizes the structural and functional dynamics of the *Bacteroides thetaiotaomicron* dextran utilisome, revealing its multi-component architecture, substrate binding mechanisms, and conformational states through integrated X-ray crystallography, calorimetry, and cryo-EM analyses.
This paper introduces CUTS, a sensitive real-time RNA biosensor that utilizes TDP-43-regulated cryptic exons to detect and quantify TDP-43 loss-of-function in neurodegenerative diseases while simultaneously demonstrating its potential for self-regulating gene replacement therapies.
RNA Polymerase I inhibition triggers NPM1 mislocalization, which initiates a coordinated histone modification cascade (H3K9ac deacetylation followed by H3K9me3 methylation) and DNA hypermethylation that collectively reshape the chromatin landscape by converting active nucleolar-associated domains into repressive lamin-associated domains and disrupting 3D nuclear architecture.
This study identifies a novel, evolutionarily primordial RAB5 binding site on the VPS15 solenoid of human VPS34 complex II, revealing that the expansion from a single ancestral binding site to two distinct sites likely enhanced membrane association and complex activity to support the development of complex eukaryotic endocytic systems.
A cross-sectional study conducted in Western Oromia, Ethiopia, between January and December 2022, revealed a 21.1% overall prevalence of pneumonic pasteurellosis-causing bacteria (*Pasteurella multocida*, *Mannheimia haemolytica*, and *Bibersteinia trehalosi*) in sheep, with significantly higher infection rates observed in clinically ill and younger animals, underscoring the need for targeted management and molecular surveillance.
This study introduces the CIERA-seq assay to demonstrate that transcription regulatory elements (promoters and enhancers) modulate gene expression through cooperative interactions that supply complementary machinery for rate-limiting steps, as well as competitive interactions for limiting resources, thereby shaping transcriptional outputs within local hubs.
This study presents the first validated assay for accurately quantifying canine mitochondrial DNA copy number in blood and brain tissue, demonstrating its potential as a novel biomarker for acute brain injury in veterinary medicine.
This paper presents a rapid, high-throughput TaqMan allelic discrimination qPCR protocol for genotyping the Muc2 p.Cys52Tyr mutation in Winnie mice using crude DNA extracts, enabling single-reaction discrimination of wild-type, heterozygous, and mutant alleles without post-amplification processing.
This paper presents a multi-modal AI/ML framework utilizing Graph Convolutional Networks to integrate global and local protein descriptors, thereby improving the accuracy of protein conformation selection and prediction for drug discovery applications.
This study demonstrates that the biophysical properties of charge and hydrophobicity, along with cooperative interactions between transcription factors, critically govern the efficiency and specificity of SOX2 and ESRRB target search dynamics within the nucleus, revealing that ESRRB relies heavily on SOX2-mediated confinement to locate its binding sites.
This study combines single-cell imaging, genetic manipulation, and polymer simulations in living *Drosophila* embryos to demonstrate that cohesin-mediated loop extrusion accelerates long-range enhancer-promoter searches through a "scan and snag" mechanism, where directional scanning facilitates diffusion-mediated tethering to ensure timely gene activation.
This year-long multi-omics study of 71 adults reveals that insulin sensitivity is a critical determinant of how the gut microbiome and plasma metabolome respond to habitual diet, with insulin-sensitive individuals showing stronger diet-omics associations that collectively improve cardiovascular risk prediction.
This study reveals that diffuse cutaneous systemic sclerosis fibroblasts survive extensive DNA damage and senescence-like features by engaging a PERK/ATF4/FOXO1 metabolic-adaptation axis that suppresses mitochondrial-dependent apoptosis, a mechanism absent in post-treatment patients and reversible through pharmacologic inhibition of PERK or FOXO1.
This study identifies CNOT6 as an essential post-transcriptional regulator that ensures healthy postnatal growth and metabolic transition by promoting the decay of Fgf21 mRNA, thereby preventing excessive FGF21 expression that would otherwise suppress anabolic metabolism and cause severe growth retardation.